has_part has part has_part has disposition at some time attenuated disposition B. melitensis cobB mutant B. suis 1330 cobB mutant PMID: 14638795 A mutant of strain Brucella suis 1330 that lacks an intact gene cobB. Information about the mutated molecule: FUNCTION: Responsible for the amidation of carboxylic groups at position A and C of either cobyrinic acid or hydrogenobrynic acid. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation (By similarity)(Swiss-Prot: Q8G020). PATHWAY: Cobalamin biosynthesis(Swiss-Prot: Q8G020). SIMILARITY: Belongs to the cobB/cobQ family. CobB subfamily(Swiss-Prot: Q8G020). MUTATION: 1,152 signature-tagged mutagenesis mutants of Brucella melitensis 16M were screened in a mouse model of infection. 36 of them to be attenuated in vivo. cobB is one of them [Ref6480:Lestrate et al., 2003]. The gene cobB from the strain Brucella suis 1330 is a virulence gene. UniProtKB accession: Q8G020 YH NCBIGene: 1166977 cobB cobyrinic acid a,c-diamide synthase