has_part has part has_part has disposition at some time attenuated disposition Brucella mutM mutant B. suis 1330 mutM mutant PMID: 14979322 A mutant of strain Brucella suis 1330 that lacks an intact gene mutM. The gene mutM from the strain Brucella suis 1330 is a virulence gene. NCBIGene: 1167886 Information about the mutated molecule: FUNCTION: Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized purines, such as 7,8-dihydro-8-oxoguanine (8-oxoG). Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates (By similarity)(Swiss-Prot: Q8FXR6). CATALYTIC ACTIVITY: Hydrolysis of DNA containing ring-opened N(7)-methylguanine residues, releasing 2,6-diamino-4-hydroxy-5-(N-methyl)formamidopyrimidine(Swiss-Prot: Q8FXR6). CATALYTIC ACTIVITY: The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate(Swiss-Prot: Q8FXR6). COFACTOR: Binds 1 zinc ion per subunit (By similarity)(Swiss-Prot: Q8FXR6). SUBUNIT: Monomer (By similarity)(Swiss-Prot: Q8FXR6). SIMILARITY: Belongs to the FPG family(Swiss-Prot: Q8FXR6). SIMILARITY: Contains 1 FPG-type zinc finger(Swiss-Prot: Q8FXR6). MUTATION: Attenuated in Differential fluorescence induction [Ref6462:Delrue et al., 2004]. UniProtKB accession: Q8FXR6 YH mutM formamidopyrimidine-DNA glycosylase