has_part has part has_part has disposition at some time attenuated disposition Brucella glnD mutant B. melitensis 16M glnD mutant Information about the mutated molecule: FUNCTION: Modifies, by uridylylation or deuridylylation the PII (glnB) regulatory protein (By similarity)(Swiss-Prot: Q8G312). CATALYTIC ACTIVITY: UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII](Swiss-Prot: Q8G312). SIMILARITY: Belongs to the glnD family(Swiss-Prot: Q8G312). MUTATION: glnD encodes for a uridylyl transferase which is the primary sensor of nitrogen. The glnD mutant via signature-tagged transposon mutagenesis is attenuated in THP1 macrophages and HeLa cells. It supports the hypothesis that the concentration of glutamine in host cells is critical for the intracellular survival of Brucella [Ref6484:Foulongne et al., 2000]. UniProtKB accession: Q8YES3 PMID: 10678941 A mutant of strain Brucella melitensis bv. 1 str. 16M that lacks an intact gene glnD. The gene glnD from the strain Brucella melitensis bv. 1 str. 16M is a virulence gene. NCBIGene: 1197515 YH BMEI1804 PII uridylyl-transferase