has_part has part has_part has disposition at some time attenuated disposition Brucella bvrS mutant B. melitensis 16M bvrS mutant Information about the mutated molecule: MUTATION: The two-component BvrSBvrR system is essential for Brucella abortus virulence. Disruption of BvrSBvrR damages the outer membrane, thus contributing to the severe attenuation manifested by bvrS and bvrR mutants. The bvrS and bvrR mutants are avirulent in mice, show reduced invasiveness to epithelial cells and macrophages, and are incapable of inhibiting lysosome fusion and replicating intracellularly [Ref6535:Manterola et al., 2005]. Mutations in the bvrR or bvrS genes hamper the penetration of B abortus in non-phagocytic cells and impairs intracellular trafficking and virulence. BvrRBvrS mutants do not recruit small GTPases of the Rho subfamily required for actin polymerization and penetration to cells. Dysfunction of the BvrRBvrS system alters the outer membrane permeability, the expression of several group 3 outer membrane proteins and the pattern of lipid A acylation. Constructs of virulent B abortus chimeras containing heterologous LPS from the bvrS(-) mutant demonstrated an altered permeability to cationic peptides similar to that of the BvrRBvrS mutants. It is hypothesized that the Brucella BvrRBvrS is a system devoted to the homeostasis of the outer membrane and, therefore in the interface for cell invasion and mounting the required structures for intracellular parasitism [Ref6536:López-Goñi et al., 2002]. In contrast to S2308 and S19, bvrS and bvrR mutant strains poorly invade HeLa cells and are rapidly targeted to cathepsin D- containing compartments [Ref6537:Pizarro-Cerdá et al., 1998]. B abortus bvrS bvrR mutants display reduced invasiveness and virulence [Ref6538:Briones et al., 2001]. Brucella bvrS and bvrR null mutants are defective in several outer membrane proteins, mainly Omp3a (former Omp25) and Omp3b as well as in the structure of the LPS molecule, but the O chain seems to be intact [Ref6466:Gorvel and Moreno, 2002]. Because bvrR and bvrS mutants are also altered in cell-surface hydrophobicity, permeability, and sensitivity to surface- targeted bactericidal peptides, it is proposed that BvrRBvrS controls cell envelope changes necessary to transit between extracellular and intracellular environments [Ref6499:Guzman-Verri et al., 2002]. BvrR/BvrS mutants are avirulent in mice, show reduced invasiveness in cells, and are unable to inhibit lysosome fusion and to replicate intracellularly [Ref6499:Guzman-Verri et al., 2002]. PMID: 16077108, 12414153, 9826346, 11401996, 12414149, 12218183 UniProtKB accession: Q8YE43 YH The gene bvrS from the strain Brucella melitensis bv. 1 str. 16M is a virulence gene. A mutant of strain Brucella melitensis bv. 1 str. 16M that lacks an intact gene bvrS. NCBIGene: 1197746 BMEI2035 SENSOR PROTEIN CHVG