has_part has part has_part has disposition at some time B. abortus hemH mutant attenuated disposition B. melitensis 16M hemH mutant NCBIGene: 1197789 A mutant of strain Brucella melitensis bv. 1 str. 16M that lacks an intact gene hemH. PMID: last step(Swiss-Prot: P0A3D7). SUBCELLULAR LOCATION: Cytoplasm (By similarity)(Swiss-Prot: P0A3D7). SIMILARITY: Belongs to the ferrochelatase family(Swiss-Prot: P0A3D7). MUTATION: A hemH knockout B. abortus mutant displayed auxotrophy for hemin, defective intracellular survival inside J774 and HeLa cells, and lack of virulence in BALBc mice. This phenotype was overcome by complementing the mutant strain with a plasmid harboring wild-type hemH. These data demonstrate that B abortus synthesizes its own heme and also has the ability to use an external source of heme [Ref6472:Almirón et al., 2001]. Information about the mutated molecule: FUNCTION: Catalyzes the ferrous insertion into protoporphyrin IX(Swiss-Prot: P0A3D7). CATALYTIC ACTIVITY: Protoporphyrin + Fe(2+) = protoheme + 2 H(+)(Swiss-Prot: P0A3D7). PATHWAY: Protoheme biosynthesis UniProtKB accession: P0A3D6 The gene hemH from the strain Brucella melitensis bv. 1 str. 16M is a virulence gene. YH hemH ferrochelatase