has_part has part has_part has disposition at some time attenuated disposition B. suis virB5 mutant B. melitensis 16M virB5 mutant NCBIGene: 1197800 The gene virB5 from the strain Brucella melitensis bv. 1 str. 16M is a virulence gene. A mutant of strain Brucella melitensis bv. 1 str. 16M that lacks an intact gene virB5. UniProtKB accession: Q8YDZ3 Information about the mutated molecule: MUTATION: A comparison of the VirB8 and VirB5 contents after induction of the B suis wild type and of virB5 and virB12 mutants further confirmed that the virB5 and virB12 genes belong to the same operon [Ref6543:Rouot et al., 2003]. Smooth strains of Brucella unable to replicate (ie, killed B suis or the avirulent mutant B suis virB5) exhibit delayed phagosome-lysosome fusion [Ref6544:Porte et al., 2003]. Polar mutations in the operon upstream of virB5 exert a greater effect on the expression of virB5 than they do on the expression of the downstream gene virB12. It indicates that in B abortus , regulatory elements other than the virB promoter may influence VirB12 protein levels [Ref6470:Sun et al., 2005]. Four independent mutants in virB5, virB9 or virB10 were highly attenuated in an in vitro infection model with human macrophages [Ref6467:O'Callaghan et al., 1999]. YH PMID: 12595417, 12595466, 16113325, 10510235 BMEII0029 VirB5