has_part has part has_part has disposition at some time B.abortus virB10 mutant attenuated disposition B. melitensis 16M virb10 mutant YH Information about the mutated molecule: MUTATION: Mutants with polar and nonpolar mutations introduced in irB10 showed different behaviors in mice and in the HeLa cell infection assay, suggesting that virB10 per se is necessary for the correct function of this type IV secretion apparatus [Ref6540:Sieira et al., 2000]. A B. abortus virB10 mutant showed a decrease of intracellular live bacteria comparable to that of the wild-type strain until 4 h after infection, indicating that a functional VirB system is not required for the short-term survival of Brucella inside macrophages. At later time points, the number of live virB10 mutants progressively decreased. Hence, the Brucella virB10 strain did not replicate, but rather was killed. Although the virB10 mutants are capable of short-term survival, they can not evade long-term degradation through fusion with lysosomes [Ref6547:Celli et al., 2003]. B abortus virB1 and virB10 mutants are unable to persist in mouse spleens after i.p. inoculation, suggest that attenuation in the animal model is due to an inability of these strains to grow intracellularly [Ref6463:Hong et al., 2000]. A B abortus virB10 mutant lost the ability to multiply in HeLa cells and was not recovered from the spleens of infected BALBc mice [Ref6538:Briones et al., 2001]. The non polar virB10 mutant was able to block the acquisition of cathepsin D, but was not able to translocate to the replication compartment [Ref6548:Boschiroli et al., 2002]. The virB10 non-polar mutants were capable of avoiding interactions with the endocytic pathway but , diverging to wild-type Brucella, were unable to reach the endoplasmic reticulum to establish their intracellular replication niche and seemed to be recycled to the cell surface [Ref6465:Comerci et al., 2001]. PMID: 10940027, 12925673, 10858227, 11401996, 12414154, 11260139 NCBIGene: 1197805 UniProtKB accession: Q8YDZ0 A mutant of strain Brucella melitensis bv. 1 str. 16M that lacks an intact gene virb10. The gene virb10 from the strain Brucella melitensis bv. 1 str. 16M is a virulence gene. BMEII0034 channel protein VIRB10-like protein