has_part has part has_part has disposition at some time B.suis miaA mutant attenuated disposition B. abortus 2308 miaA mutant Information about the mutated molecule: FUNCTION: Catalyzes the first step in the biosynthesis of 2-methylthio-N6-(delta(2)-isopentenyl)-adenosine (MS[2]I[6]A) adjacent to the anticodon of several tRNA species (By similarity)(Swiss-Prot: Q8CY40). CATALYTIC ACTIVITY: Isopentenyl diphosphate + tRNA = diphosphate + tRNA containing 6-isopentenyladenosine(Swiss-Prot: Q8CY40). SIMILARITY: Belongs to the IPP transferase family(Swiss-Prot: Q8CY40). MUTATION: A study with miniTn5 mutants of B. suis constitutively expressing gfp indicates that B. suis miaA gene is required for intracellular multiplication in human macrophage THP-1 cells [Ref412:Kohler et al., 2002]. A mutant of strain Brucella melitensis biovar Abortus 2308 that lacks an intact gene miaA. YH NCBIGene: 3787973 UniProtKB accession: Q2YS31 The gene miaA from the strain Brucella melitensis biovar Abortus 2308 is a virulence gene. PMID: 12438693 miaA tRNA delta(2)-isopentenylpyrophosphate transferase