has_part has part has_part has disposition at some time attenuated disposition B. abortus znuA mutant B. abortus 2308 znuA mutant The gene znuA from the strain Brucella melitensis biovar Abortus 2308 is a virulence gene. UniProtKB accession: Q2YJH5 Information about the mutated molecule: MUTATION: Brucella abortus znuA mutant via mini-Tn5Km2 transposon mutagenesis has intracellular growth defect inside HeLa cells [Ref6469:Kim et al., 2003]. High-affinity zinc uptake system protein mutant (znuA mutant) showed reduced growth in zinc chelated medium, and failed to replicate in HeLa cells and mouse bone marrow-derived macrophages. Transformation of znuA mutant with a shuttle vector pBBR1MCS-4 containing znuA gene restored the growth in zinc chelated medium and intracellular replication in HeLa cells and macrophages to a level comparable to that of wild-type strain. Bacterial internalization into HeLa cells and macrophages and co-localization with either late endosomes or lysosomes of znuA mutant were not different from those of wild-type strain. These results suggest that znuA does not contribute to intracellular trafficking of B abortus, but contributes to utilization of zinc required for intracellular growth [Ref6469:Kim et al., 2003]. YH NCBIGene: 3827700 PMID: 12761078 A mutant of strain Brucella melitensis biovar Abortus 2308 that lacks an intact gene znuA. znuA Periplasmic solute binding protein