bearer of at some time only_in_taxon Brucella virulence factor disposition Brucella suis 1330 hemH protein ferrochelatase NCBIProteinGI: 23499843 This protein is a Brucella virulence factor. FUNCTION: Catalyzes the ferrous insertion into protoporphyrin IX(Swiss-Prot: P0A3D7). CATALYTIC ACTIVITY: Protoporphyrin + Fe(2+) = protoheme + 2 H(+)(Swiss-Prot: P0A3D7). PATHWAY: Protoheme biosynthesis LocusTag: BRA0076 NCBIGene: 1164513 NCBIProteinAccess:NP_699283.1 UniProtKB: PR:P0A3D7 Gene name: hemH PMID: last step(Swiss-Prot: P0A3D7). SUBCELLULAR LOCATION: Cytoplasm (By similarity)(Swiss-Prot: P0A3D7). SIMILARITY: Belongs to the ferrochelatase family(Swiss-Prot: P0A3D7). MUTATION: A hemH knockout B. abortus mutant displayed auxotrophy for hemin, defective intracellular survival inside J774 and HeLa cells, and lack of virulence in BALBc mice. This phenotype was overcome by complementing the mutant strain with a plasmid harboring wild-type hemH. These data demonstrate that B abortus synthesizes its own heme and also has the ability to use an external source of heme [Ref6472:Almirón et al., 2001]. Molecule Role Annotation: FUNCTION: Catalyzes the ferrous insertion into protoporphyrin IX(Swiss-Prot: P0A3D7). CATALYTIC ACTIVITY: Protoporphyrin + Fe(2+) = protoheme + 2 H(+)(Swiss-Prot: P0A3D7). PATHWAY: Protoheme biosynthesis