bearer of at some time only_in_taxon Brucella virulence factor disposition Brucella melitensis bv. 1 str. 16M BMEI0480 protein peptidyl-tRNA hydrolase LocusTag: BMEI0480 PMID: 15385478 This protein is a Brucella virulence factor. FUNCTION: The natural substrate for this enzyme may be peptidyl-tRNAs which drop off the ribosome during protein synthesis (By similarity)(Swiss-Prot: P65864). CATALYTIC ACTIVITY: N-substituted aminoacyl-tRNA + H(2)O = N-substituted amino acid + tRNA(Swiss-Prot: P65864). SUBUNIT: Monomer (By similarity)(Swiss-Prot: P65864). SUBCELLULAR LOCATION: Cytoplasm (By similarity)(Swiss-Prot: P65864). SIMILARITY: Belongs to the PTH family(Swiss-Prot: P65864). MUTATION: pth encodes for a peptidyl tRNA hydrolase. Transposon insertion in pth has a polar effect on dugA expression and that the pth/dugA mutant is deficient in iron assimilation because of altered expression of the dugA gene. Only minor difference in intracellular growth in bovine macrophages and HeLa cells between the pth/dugA mutant and wild-type strains was observed [Ref6498:Danese et al., 2004]. NCBIProteinGI: 17986763 NCBIProteinAccess:NP_539397.1 Molecule Role Annotation: FUNCTION: The natural substrate for this enzyme may be peptidyl-tRNAs which drop off the ribosome during protein synthesis (By similarity)(Swiss-Prot: P65864). CATALYTIC ACTIVITY: N-substituted aminoacyl-tRNA + H(2)O = N-substituted amino acid + tRNA(Swiss-Prot: P65864). SUBUNIT: Monomer (By similarity)(Swiss-Prot: P65864). SUBCELLULAR LOCATION: Cytoplasm (By similarity)(Swiss-Prot: P65864). SIMILARITY: Belongs to the PTH family(Swiss-Prot: P65864). MUTATION: pth encodes for a peptidyl tRNA hydrolase. Transposon insertion in pth has a polar effect on dugA expression and that the pth/dugA mutant is deficient in iron assimilation because of altered expression of the dugA gene. Only minor difference in intracellular growth in bovine macrophages and HeLa cells between the pth/dugA mutant and wild-type strains was observed [Ref6498:Danese et al., 2004]. Gene name: pth NCBIGene: 1196191 UniProtKB: PR:P65863