bearer of at some time only_in_taxon Brucella virulence factor disposition Brucella melitensis biovar Abortus 2308 sodC protein Copper/Zinc superoxide dismutase UniProtKB: PR:Q2YKV9 Gene name: sodC Molecule Role Annotation: FUNCTION: Destroys radicals which are normally produced within the cells and which are toxic to biological systems (By similarity)(Swiss-Prot: P66827). CATALYTIC ACTIVITY: 2 superoxide + 2 H(+) = O(2) + H(2)O(2)(Swiss-Prot: P66827). COFACTOR: Binds 1 copper ion per subunit (By similarity)(Swiss-Prot: P66827). COFACTOR: Binds 1 zinc ion per subunit (By similarity)(Swiss-Prot: P66827). SUBUNIT: Homodimer (By similarity)(Swiss-Prot: P66827). SUBCELLULAR LOCATION: Periplasmic (By similarity)(Swiss-Prot: P66827). SIMILARITY: Belongs to the Cu-Zn superoxide dismutase family(Swiss-Prot: P66827). IMMUNOGENICITY: Induces antigen-specific Th1 immune response, as indicated by the specific induction of serum IgG2a, but not IgG1, antibodies and by the secretion of IFN-?, but not IL-4, by the Cu/Zn SOD-stimulated splenocytes. Has been used for vaccine development [Ref6552:Vemulapalli et al., 2000][Ref6552:Vemulapalli et al., 2000][Ref6552:Vemulapalli et al., 2000]. MUTATION: An isogenic sodC mutant constructed from B abortus 2308 by gene replacement exhibited much greater susceptibility to killing by exogenous O(2)(-) than the parental 2308 strain, supporting a role for SodC in protecting this bacterium from O(2)(-) stress. The B abortus sodC mutant was much more sensitive to killing by cultured resident peritoneal macrophages from C57BL6J mice than 2308, and its attenuation in cultured murine macrophages was enhanced when these phagocytes were treated with gamma interferon. The attenuation of the B abortus sodC mutant in both resting and IFN-gamma -activated macrophages was alleviated in the presence of the NADPH oxidase inhibitor apocynin. Consistently, the B abortus sodC mutant also displayed significant attenuation in infected C57BL6J mice compared to the parental strain. These findings suggest that SodC protects B abortus 2308 from the respiratory burst of host macrophages [Ref6552:Vemulapalli et al., 2000]. PMID: 10816475 NCBIProteinGI: 83269434 LocusTag: BAB2_0535 NCBIGene: 3827840 NCBIProteinAccess:YP_418725.1 This protein is a Brucella virulence factor. FUNCTION: Destroys radicals which are normally produced within the cells and which are toxic to biological systems (By similarity)(Swiss-Prot: P66827). CATALYTIC ACTIVITY: 2 superoxide + 2 H(+) = O(2) + H(2)O(2)(Swiss-Prot: P66827). COFACTOR: Binds 1 copper ion per subunit (By similarity)(Swiss-Prot: P66827). COFACTOR: Binds 1 zinc ion per subunit (By similarity)(Swiss-Prot: P66827). SUBUNIT: Homodimer (By similarity)(Swiss-Prot: P66827). SUBCELLULAR LOCATION: Periplasmic (By similarity)(Swiss-Prot: P66827). SIMILARITY: Belongs to the Cu-Zn superoxide dismutase family(Swiss-Prot: P66827). IMMUNOGENICITY: Induces antigen-specific Th1 immune response, as indicated by the specific induction of serum IgG2a, but not IgG1, antibodies and by the secretion of IFN-?, but not IL-4, by the Cu/Zn SOD-stimulated splenocytes. Has been used for vaccine development [Ref6552:Vemulapalli et al., 2000][Ref6552:Vemulapalli et al., 2000][Ref6552:Vemulapalli et al., 2000]. MUTATION: An isogenic sodC mutant constructed from B abortus 2308 by gene replacement exhibited much greater susceptibility to killing by exogenous O(2)(-) than the parental 2308 strain, supporting a role for SodC in protecting this bacterium from O(2)(-) stress. The B abortus sodC mutant was much more sensitive to killing by cultured resident peritoneal macrophages from C57BL6J mice than 2308, and its attenuation in cultured murine macrophages was enhanced when these phagocytes were treated with gamma interferon. The attenuation of the B abortus sodC mutant in both resting and IFN-gamma -activated macrophages was alleviated in the presence of the NADPH oxidase inhibitor apocynin. Consistently, the B abortus sodC mutant also displayed significant attenuation in infected C57BL6J mice compared to the parental strain. These findings suggest that SodC protects B abortus 2308 from the respiratory burst of host macrophages [Ref6552:Vemulapalli et al., 2000].