bearer of at some time only_in_taxon Brucella virulence factor disposition Brucella melitensis bv. 1 str. 16M miaA protein tRNA delta(2)-isopentenylpyrophosphate transferase NCBIGene: 1196327 NCBIProteinGI: 17986899 This protein is a Brucella virulence factor. FUNCTION: Catalyzes the first step in the biosynthesis of 2-methylthio-N6-(delta(2)-isopentenyl)-adenosine (MS[2]I[6]A) adjacent to the anticodon of several tRNA species (By similarity)(Swiss-Prot: Q8CY40). CATALYTIC ACTIVITY: Isopentenyl diphosphate + tRNA = diphosphate + tRNA containing 6-isopentenyladenosine(Swiss-Prot: Q8CY40). SIMILARITY: Belongs to the IPP transferase family(Swiss-Prot: Q8CY40). MUTATION: A study with miniTn5 mutants of B. suis constitutively expressing gfp indicates that B. suis miaA gene is required for intracellular multiplication in human macrophage THP-1 cells [Ref412:Kohler et al., 2002]. UniProtKB: PR:Q8YI29 Gene name: miaA LocusTag: BMEI0616 PMID: 12438693 NCBIProteinAccess:NP_539533.1 Molecule Role Annotation: FUNCTION: Catalyzes the first step in the biosynthesis of 2-methylthio-N6-(delta(2)-isopentenyl)-adenosine (MS[2]I[6]A) adjacent to the anticodon of several tRNA species (By similarity)(Swiss-Prot: Q8CY40). CATALYTIC ACTIVITY: Isopentenyl diphosphate + tRNA = diphosphate + tRNA containing 6-isopentenyladenosine(Swiss-Prot: Q8CY40). SIMILARITY: Belongs to the IPP transferase family(Swiss-Prot: Q8CY40). MUTATION: A study with miniTn5 mutants of B. suis constitutively expressing gfp indicates that B. suis miaA gene is required for intracellular multiplication in human macrophage THP-1 cells [Ref412:Kohler et al., 2002].