has_part
has part
has_part
has disposition at some time
B. abortus hfq mutant
attenuated disposition
B. abortus 2308 hfq mutant
The gene hfq from the strain Brucella melitensis biovar Abortus 2308 is a virulence gene.
UniProtKB accession: Q2YPW9
Information about the mutated molecule: FUNCTION: RNA-binding protein that stimulates the elongation of poly(A) tails (By similarity)(Swiss-Prot: P0A3G8). SIMILARITY: Belongs to the hfq family(Swiss-Prot: P0A3G8). MUTATION: hfq encodes for the RNA binding protein host factor I (HF-I). The hfq knock out strain has been showed a reduced growth rate and is unable to utilize glucose as a sole carbon source[Ref6495:Sonnleitner et al., 2003]. hfq is required for the efficient translation of the stationary-phase sigma factor RpoS in many bacteria, and a Brucella abortus hfq mutant displays a phenotype in vitro, which suggests that it has a generalized defect in stationary-phase physiology. The inability of the B. abortus hfq mutant to survive and replicate in a wild-type manner in cultured murine macrophages, and the profound attenuation displayed by this strain and its B melitensis counterpart in experimentally infected animals indicate that stationary -phase physiology plays an essential role in the capacity of the brucellae to establish and maintain long-term intracellular residence in host macrophages [Ref6532:Roop et al., 2003]. In contrast to B abortus 2308, the isogenic hfq and bacA mutants remained in acidic, LAMP-1 phagosomes and failed to initiate intracellular replication [Ref6532:Roop et al., 2003]. A hfq mutant of B abortus was eliminated from mouse spleens more rapidly than the wild type [Ref6532:Roop et al., 2003].
NCBIGene: 3788748
PMID: 14521880, 12730323
YH
A mutant of strain Brucella melitensis biovar Abortus 2308 that lacks an intact gene hfq.
hfq
RNA-binding protein Hfq