bearer of at some time
only_in_taxon
Brucella virulence factor disposition
Brucella suis 1330
hemH
protein
ferrochelatase
NCBIProteinGI: 23499843
This protein is a Brucella virulence factor. FUNCTION: Catalyzes the ferrous insertion into protoporphyrin IX(Swiss-Prot: P0A3D7). CATALYTIC ACTIVITY: Protoporphyrin + Fe(2+) = protoheme + 2 H(+)(Swiss-Prot: P0A3D7). PATHWAY: Protoheme biosynthesis
LocusTag: BRA0076
NCBIGene: 1164513
NCBIProteinAccess:NP_699283.1
UniProtKB: PR:P0A3D7
Gene name: hemH
PMID: last step(Swiss-Prot: P0A3D7). SUBCELLULAR LOCATION: Cytoplasm (By similarity)(Swiss-Prot: P0A3D7). SIMILARITY: Belongs to the ferrochelatase family(Swiss-Prot: P0A3D7). MUTATION: A hemH knockout B. abortus mutant displayed auxotrophy for hemin, defective intracellular survival inside J774 and HeLa cells, and lack of virulence in BALBc mice. This phenotype was overcome by complementing the mutant strain with a plasmid harboring wild-type hemH. These data demonstrate that B abortus synthesizes its own heme and also has the ability to use an external source of heme [Ref6472:Almirón et al., 2001].
Molecule Role Annotation: FUNCTION: Catalyzes the ferrous insertion into protoporphyrin IX(Swiss-Prot: P0A3D7). CATALYTIC ACTIVITY: Protoporphyrin + Fe(2+) = protoheme + 2 H(+)(Swiss-Prot: P0A3D7). PATHWAY: Protoheme biosynthesis