bearer of at some time
only_in_taxon
Brucella virulence factor disposition
Brucella melitensis biovar Abortus 2308
hemH
protein
ferrochelatase
LocusTag: BAB2_0075
Molecule Role Annotation: FUNCTION: Catalyzes the ferrous insertion into protoporphyrin IX(Swiss-Prot: P0A3D7). CATALYTIC ACTIVITY: Protoporphyrin + Fe(2+) = protoheme + 2 H(+)(Swiss-Prot: P0A3D7). PATHWAY: Protoheme biosynthesis
Gene name: hemH
NCBIProteinAccess:YP_418320.1
NCBIGene: 3828247
NCBIProteinGI: 83269029
PMID: last step(Swiss-Prot: P0A3D7). SUBCELLULAR LOCATION: Cytoplasm (By similarity)(Swiss-Prot: P0A3D7). SIMILARITY: Belongs to the ferrochelatase family(Swiss-Prot: P0A3D7). MUTATION: A hemH knockout B. abortus mutant displayed auxotrophy for hemin, defective intracellular survival inside J774 and HeLa cells, and lack of virulence in BALBc mice. This phenotype was overcome by complementing the mutant strain with a plasmid harboring wild-type hemH. These data demonstrate that B abortus synthesizes its own heme and also has the ability to use an external source of heme [Ref6472:Almirón et al., 2001].
This protein is a Brucella virulence factor. FUNCTION: Catalyzes the ferrous insertion into protoporphyrin IX(Swiss-Prot: P0A3D7). CATALYTIC ACTIVITY: Protoporphyrin + Fe(2+) = protoheme + 2 H(+)(Swiss-Prot: P0A3D7). PATHWAY: Protoheme biosynthesis
UniProtKB: PR:Q2YIS9