has characteristic continuous variable biological state optimization method Enzyme Cost Minimization 2022-03-27 http://identifiers.org/doi:10.1371/journal.pcbi.1005167 https://gitlab.com/equilibrator/metabolic-pathway-analysis ECM EN For a given metabolic network model, the Enzyme Cost Minimization method determines plausible metabolite and enzyme concentrations (which determine thermodynamic forces and enzyme catalytic rates). Fluxes, enzyme kinetic constants, admissible metabolite concentration ranges, and enzyme cost weights (e.g. enzyme molecular masses) are given as input data. The method applies the principle that high enzyme (or enzyme plus metabolite) concentrations must be avoided, and maximizes a weighted sum of enzyme and metabolite concentrations. JRK WL metabolic system