has characteristic continuous variable biological state optimization method Max-min Driving Force method 2022-03-27 http://identifiers.org/doi:10.1371/journal.pcbi.1003483 https://gitlab.com/equilibrator/metabolic-pathway-analysis EN For a given metabolic network model, the MDF method determines plausible metabolite concentrations and thermodynamic forces. Flux directions, equilibrium constants (or equivalently, standard Gibbs free energies of reactions) and admissible metabolite concentration ranges are given as input data. The method applies the principle that low thermodynamic forces must be avoided, and maximizes the minimum thermodynamic force across the entire network. JRK MDF WL metabolic system