is rich medium for synH medium an artificial medium with a defined chemical composition designed to mimic AFEX-pretreated corn stover hydrolysate (ACSH) synthetic hydrolisate colombos:MEDIUM.SynH Water (∼700 ml) 6.25 ml of 1.6 M KPO4 buffer pH 7.2 20 ml of 1.5 M ammonium sulfate 20 ml of 2.25 M KCl 1.25 M NaCl 20 ml of a 50X amino acid stock (except tyrosine) 20 ml of 8.75 mM tyrosine dissolved in 50 mM HCl 50 ml of 1 mM each adenine, guanine, cytosine and uracil dissolved in 10 mM KOH 10 ml of vitamin stock (1 mM each thiamine, calcium pantothenate, p-aminobenzoic acid, p- hydroxybenzoic acid, and 2,3-dihydroxybenzoic acid) 1 ml of a 1000X stock of micronutrients (ZnCl2 , MnCl2 , CuCl2 , CoCl2 , H3 BO3 , (NH4 )6 Mo7 O24 , and FeCl3 ) 1 ml of 1 M magnesium chloride 1ml of 90mM CaCl2 10ml of 1M sodium formate 10mM sodium nitrate 50 mM sodium succinate 1 ml of 3 M glycerol 1 ml of 500 mM lactic acid 1 ml of 700 mM glycine betaine 700 mM choline chloride 200 mM DL-carnitine (osmolytes) 5.61 g acetamide 2.71 g sodium acetate 3.3 g sodium pyruvate 2.94 g sodium citrate 1.34 g DL-malic acid 60 g D-glucose 30 g D-xylose 5.1 g D-arabinose 1.48 g D-fructose 1.15 g D- galactose 468 mg D-mannose After adjusting to pH 7 with 10 N NaOH, the final volume is adjusted to 1L. PMID: 25177315 microbiological culture medium Escherichia coli K-12