microbiological medium ingredient, derived from chemical hydrolysis of protein casamino acids Carrine Blank An acid hydrolysate of casein (milk protein from cow's milk, Bos taurus), used for the culturing of microorganisms. acid digest of casein Casein Hydrolysate From BD Bionutrients Technical Manual (3rd edition revised): Bacto™ Casamino Acids is an acid hydrolysate of casein, prepared according to the method described by Mueller and Miller. The method described, reduces the sodium chloride and iron content of the hydrolyzed casein. This hydrolyzed casein, supplemented with inorganic salts, growth factors, cystine, maltose and an optimum amount of iron was used by Mueller and Miller to prepare diptheria toxin. Bacto Casamino Acids duplicate this specially treated hydrolyzed casein. Ash content is 18.3% NaCl content is 12.1%