is rich medium for microbiological culture medium LB medium An organic-rich, liquid microbiological culture medium containing tryptone, yeast extract, and sodium chloride. Used to cultivate Escherichia coli. LB broth From: LB Broth, Miller (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use LB Agar, Miller and LB Broth, Miller (Luria-Bertani) are used for maintaining and propagating Escherichia coli in molecular microbiology procedures. Principles of the Procedure Peptone provides nitrogen and carbon. Vitamins (including B vitamins) and certain trace elements are provided by yeast extract. Sodium ions for transport and osmotic balance are provided by sodium chloride. Agar is the solidifying agent in LB Agar, Miller. Formulae Difco™ LB Agar, Miller Approximate Formula* Per Liter Tryptone.................................................................... 10.0 g Yeast Extract................................................................ 5.0 g Sodium Chloride........................................................ 10.0 g Agar.......................................................................... 15.0 g Difco™ LB Broth, Miller Consists of the same ingredients without the agar. *Adjusted and/or supplemented as required to meet performance criteria. pH 7.0 ± 0.2 Luria broth Carrine Blank Escherichia coli K-12