is minimal medium for microbiological culture medium Escherichia coli K-12 DSMZ Medium 382 http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium382.pdf 382. MINERAL MEDIUM M9 for E. coli JM strains 10 x M9 salts (see below) 100.0 ml 1 M MgSO4 1.0 ml 0.1 M CaCl2 1.0 ml 1 M Thiamine-HCl x 2 H2O (filter-sterilized) 1.0 ml Glucose (20%) 10.0 ml Proline 20.0 mg Distilled water 900.0 ml The above solutions should be sterilized separately by filtration (thiamine, glucose) or autoclaving. 10 x M9 salts (per l): Na2HPO4 60.0 g KH2PO4 30.0 g NH4Cl 10.0 g NaCl 5.0 g Adjust pH to 7.4. © 2007 DSMZ GmbH - All rights reserved Carrine Blank DSM strains: Minimal medium M9 M9 minimal medium An organic-rich, liquid culture medium comprised of 10 x M9 salts, magnesium sulfate, calcium chloride, thiamine hydrochloride, glucose, and proline.