phenotype-based detection assay nuclear translocation assay PSI-MI orchard Method to detect interaction by inducing nuclear localization of one participant, which would then pull an interacting participant along with it into the nucleus. As both participants are labeled, the difference in nuclear localization between the induced and non-induced states provides an indication of the interaction between the two molecules. MI:1089 2011-07-05T11:27:16Z nuclear translocation