protein complementation assay split luciferase complementation 2012-06-07T04:56:59Z Two proteins of interest, a bait and prey, which are genetically fused to amino- and carboxy-terminal fragments of luciferase, are transiently expressed. Physical interactions of these bait and prey proteins reconstitute some of the luciferase activity and result in light emission in the presence of the luciferase substrate. orchard PSI-MI MI:1203