split luciferase complementation split firefly luciferase complementation orchard MI:1204 PSI-MI Two proteins of interest, a bait and prey, which are genetically fused to amino- and carboxy-terminal fragments of firefly (Photinus pyralis) luciferase, are transiently expressed. Physical interactions of these bait and prey proteins reconstitute some of the luciferase activity and result in light emission in the presence of the luciferase substrate. 2012-06-07T05:05:36Z