ire1 reconstruction orchard ER-MYTH PSI-MI Upon interaction of N-terminal generated Ire1p-fusions, the Ire1p kinase domains oligomerize, transphosphorylate and activate their C-terminal RNAseL domains, which specifically splice the mRNA of a transcriptional activator, Hac1. The expression of the mature form of Hac1p leads to the interaction-specific expression of a reporter. Specifically designed to identify interactors of proteins found in the endoplasmic reticulum. 2014-01-08T10:17:38Z MI:1321 endoplasmic reticulum membrane yeast-two hybrid membrane two hybrid