luminiscence technology bimolecular luminiscence complementation bioluminescence complementation bioluminescence complementation assay 2015-04-29T12:03:27Z PSI-MI luminiscence complementation split-luciferase complementation assay MI:2170 The bimolecular luminescence complementation (BiLC) is an assay for determination of protein interactions and/or their location in living cells. This approach is based on complementation between two fragments of a bioluminescent enzyme such as luciferin or aequorin. Interactions between proteins fused to each fragment bring the fragments together resulting in the reconstitution of a fully functional bioluminescent enzyme that can be identified through microscopy or luminescence detection. luminescence complementation assay split-luciferase complementation ppm bilc