nucleic acid uv cross-linking assay crac ppm MI:2194 cross-linking and analysis of cDNAs crac PSI-MI 2015-06-15T11:26:33Z Combination of UV cross-linking and affinity purification where the protein of interest bears a tag used for pull-down or immunoprecipitation. As in CLIP, unspecific proteins are digested via proteinase K treatment and RNAs are tagged with oligonucleotide linkers. RNA can be then identified via Northern blotting or using RT-PCR and then sequencing of the generated cDNA. CRAC