two hybrid bait and prey pooling approach barcode fusion genetics two hybrid This method allows screening of a full matrix of protein pairs in a single multiplexed strain pool. A doubly engineered clone pool is prepared so that each clone bears two distinct DNA barcodes flanked by site specific recombination sequences. Positive bait-prey combinations allow activation of reporter genes and their respective barcodes undergo recombination, creating unique barcode combinations. Recombined barcode tags are then fused, extracted and sequenced for identification of interacting pairs. BGF-2h 2016-05-05T14:33:32Z ppm bfg-2h bfg two hybrid PSI-MI MI:2215 barcode fusion genetics two hybrid