two hybrid bait and prey pooling approach Cr-two hybrid MI:2277 PSI-MI cr-two hybrid ppm Cre recombinase two hybrid This method uses Cre recombinase as a two-hybrid protein-protein interaction reporter that functions intracellularly to covalently and unidirectionally link interacting bait and prey plasmids via specialized loxP sites that flank the protein-coding sequences. The linked protein-coding sequences serve as interaction-identifying DNA molecules that enable massively multiplexed screening coupled with next-generation DNA sequencing to detect protein-protein interactions. 2017-06-20T15:57:41Z cr-2h