far western blotting southwestern blotting southwestern blot ppm PSI-MI 2017-11-15T15:35:20Z MI:2283 southwestern blotting Southwestern blotting, is a lab technique which involves identifying and characterizing DNA-binding proteins by their ability to bind to specific oligonucleotide probes, generally radioactively labeled. The proteins are separated by gel electrophoresis and are subsequently transferred to nitrocellulose membranes similar to other types of blotting.