post transcriptional interference miRNA interference luciferase reporter assay MI:2285 miRNA interference luciferase assay pporras The method is based on the functional effect of the binding of the microRNA on the target (mRNA or LncRNA), which is the repression of the expression of the target. To validate a sequence as a direct target of a microRNA, a luciferase reporter gene carrying the wt candidate sequence or its mutated form is used, and the expression of the target is evaluated with a luciferase assay. If the wt is significantly less expressed than the mutant, the binding occurs. 2018-05-02T15:22:12Z PSI-MI