high-performance liquid chromatography 2013-06-20T16:54:33Z A high pressure form of liquid column chromatography in which the solvent is forced through the column under high pressures of up to 400 atmospheres rather than being allowed to drip through the column under gravity. Liquid chomatography is a method of separating and identifying the components of a complex mixture by differential movement through a two-phase system, in which the movement is effected by a flow of a liquid (mobile phase) which percolates through an adsorbent (stationary phase). HPLC JSmith MMO:0000394 high-pressure liquid chromatography measurement_method_ontology liquid column chromatography