enzyme linked immunosorbent assay enzyme-linked immunosorbent spot assay 2014-01-14T12:55:54Z A modified version of the ELISA (enzyme-linked immunosorbent assay) protocol in which viable cells are plated onto membranes treated with antibodies against the molecule of interest. Analyte secreted from a cell is immediately adsorbed onto the membrane before dilution or difusion and membranes are processed as in the ELISA protocol, yielding spots represending individual activated or responding cells. ELISPOT assay JSmith MMO:0000458 measurement_method_ontology