enzymatic method polymerase chain reaction assay 2014-01-14T13:21:30Z A rapid technique for in vitro amplification of specific DNA sequences, allowing small quantities of short sequences to be analyzed without cloning. DNA sequences are amplified in vitro by separating the DNA into two strands and incubating it in a series of temperature cycles with oligonucleotide primers and heat-stable DNA polymerase. JSmith MMO:0000459 PCR PCR assay measurement_method_ontology transcript expression assay