quantitative reverse transcription polymerase chain reaction 2014-01-14T13:28:06Z JSmith MMO:0000462 Real-time PCR in which a sample of RNA is first reverse transcribed to convert the RNA into complementary DNA (cDNA) and then a quantitative real-time PCR reaction is carried out on the resulting sample. measurement_method_ontology qRT-PCR qRT-PCR quantitative RT-PCR real-time RT-PCR true quantitative reverse transcription polymerase chain reaction assay