high-performance liquid chromatography mass spectrometry high-performance liquid chromatography-mass spectrometry 2014-06-05T15:09:27Z An analytical technique in that combines the physical separation capabilities of high pressure liquid chromatography (HPLC) with the mass analysis capabilities of mass spectrometry (MS). Molecular mixtures are initially separated into components by HPLC. As these components exit the column, they are subjected to mass spectrometry for identification based on their mass-to-charge ratio and behavior in a magnetic field. HPLC-MS JSmith MMO:0000537 high pressure liquid chromatography-mass spectrometry measurement_method_ontology