plate count using selective/restrictive growth conditions plate count using supplemented brain-heart infusion/vancomycin plates 2015-05-04T12:44:02Z A plate colony counting method that uses supplemented brain-heart infusion (sBHI) agar plates to which hemin/heme, nicotinamide adenine dinucleotide (NAD) and the antibiotic vancomycin have been added. SBHI/vancomycin plates support the growth of fastidious bacteria with an obligate requirement for heme and beta-NAD such as various strains of Haemophilus influenzae, and prevent the growth of vancomycin-sensitive bacteria such as Streptococcus pneumoniae. JSmith MMO:0000566 colony count using sBHI/vancomycin plates measurement_method_ontology plate count using SBHI/vancomycin plates