real-time polymerase chain reaction assay reverse transcription polymerase chain reaction assay quantitative reverse transcription polymerase chain reaction assay 2018-05-28T18:35:12Z A method in which amplification of a specific RNA/cDNA sequence by a reverse transcription polymerase chain reaction is monitored by detection of fluorescent probes or intercalating dyes that indicate the (absolute or relative) amount of product being produced in real time. ECO:0000231 MMO:0000462 MMO:0000656 OBI:0000911 RT-qPCR jrsmith measurement_method_ontology qRT-PCR quantitative RT-PCR quantitative reverse transcription polymerase chain reaction real-time RT-PCR