multiphoton microscopy 2021-01-15T13:32:33Z MMO:0000742 MPM Multiphoton microscopy (MPM) is an imaging technique that allows intact biological samples to be visualized at high resolution. MPM is based on nonlinear optical processes such as two-photon and three-photon excited fluorescence (2PEF, 3PEF) and second and third harmonic generation (SHG, THG). Most commonly, 2PEF and 3PEF are used to image fluorescent dyes or endogenous molecules. PMID:25680709 measurement_method_ontology slaulede in vivo microscopy