liquid column chromatography affinity chromatography 2022-05-26T15:41:19Z AC Affinity chromatography is a type of gel filtration chromatography which separates proteins on the basis of a reversible interaction between a protein (or group of proteins) and a specific ligand coupled to a chromatography matrix. With such high selectivity and hence high resolution for the protein(s) of interest, purification levels in the order of several thousand-fold with high recovery of active material are achievable. Samples are concentrated during binding and the target protein(s) is collected in a purified, concentrated form. Examples include antibody/antigen, enzyme/substrate, and enzyme/inhibitor interactions. MMO:0000845 PMID:15780096 measurement_method_ontology slaulede