RNA-seq assay single nucleus RNA-seq assay 2026-02-27T16:07:40Z MMO:0000869 This is a method which assesses transcript expression and structure in single cell nuclei. Following tissue processing and isolation of nuclei, sample RNA is purified, fragmented, reverse transcribed to cDNA with the addition of sequencing primers, sequenced and analyzed. Unlike single cell RNA-seq, this method does not require viable, intact cells and can be used on frozen or archived samples. caripark measurement_method_ontology sNuc-seq assay single nuclei RNA-seq assay single nucleus RNA sequencing assay snRNA-seq assay