iTRAQ8plex-116 reporter+balance reagent acylated residue iTRAQ8plex-116 reporter+balance reagent N6-acylated lysine 304.205352783203125 304.209991455078125 432.300323486328125 432.30999755859375 H 24 (12)C 7 (13)C 7 (14)N 3 (15)N 1 (16)O 3 MOD:01551 The reagent consists of a reporter group, a balance group and a protein reactive N-oxysuccinimide group. The reporter group, an isotopically labeled 1,4-dimethylpiperazine, is connected to a balance group that is isotopically labeled to produce a nominally isobaric combined modification. Representative mass and accurate mass for 113, 114, 116 & 117 none (4-methylpiperazin-1-yl)acetyl Applied Biosystems iTRAQ(TM) multiplexed quantitation chemistry iTRAQ8plex:13C(7)15N(1) artifact PSI-MOD H 36 (12)C 13 (13)C 7 (14)N 5 (15)N 1 (16)O 4 A protein modification that effectively replaces the N6'-hydrogen atom of a lysine residue with the Applied Biosystems iTRAQ8plex-116 reporter+balance group. K iTRAQ8plex iTRAQ8plex reporter+balance reagent N-acylated residue N6-acylated L-lysine