Immunological Flow Cytometry Intracellular Cytokine Stain Flow Cytometric Assay Intracellular Cytokine Stain Flow Cytometric Assay CDISC INTRACELLULAR CYTOKINE STAIN FLOW CYTOMETRIC ASSAY C178026 Laboratory Procedure Intracellular Cytokine Staining CL1647977 ICS A technique that is used with flow cytometry to measure cytokine production in isolated cells. Cells are stimulated with an antigen or mitogen, and cytokine secretion is blocked using protein transport inhibitors. The cells are then stained with fluorochrome labeled monoclonal antibodies that target surface markers, fixed and permeabilized, and stained with fluorochrome labeled anti-cytokine monoclonal antibodies prior to analysis by flow cytometry. Intracellular Cytokine Stain Flow Cytometric Assay A technique that is used with flow cytometry to measure cytokine production in isolated cells. Cells may be stimulated with an antigen or mitogen, and cytokine secretion may be blocked using protein transport inhibitors. The cells are then stained with fluorochrome labeled monoclonal antibodies that target surface markers, fixed and permeabilized, and stained with fluorochrome labeled anti-cytokine monoclonal antibodies prior to analysis by flow cytometry. Clinical Data Interchange Standards Consortium Terminology CDISC SDTM Terminology Cytokine Measurement CDISC SDTM Method Terminology