RNA-seq assay polyA-depleted RNA sequencing assay An RNA-seq assay to study non-polyadenylated transcripts, such as, but not exclusive to unspliced and circular isoforms, through the depletion of polyadenylated transcripts prior to high-throughput sequencing. url:http://www.tandfonline.com/doi/full/10.1080/15476286.2015.1110676 polyA-depleted RNA sequencing assay ENCODE Jason Hilton polyA-depleted RNA-seq Mark A. Miller url:http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0030733