has part realizes has specified input achieves_planned_objective role of has role evaluant role polymerase chain reaction DNA extract insecticide resistance detection objective polymerase chain reaction assay insecticide resistance by monitoring known mutations assay insecticide resistance by PCR amplification of specific alleles assay allele-specific amplification and amplification refractory mutation system insecticide resistance by PCR amplification of specific alleles assay PASA PCR amplification of specific alleles VEuPathDB https://github.com/obi-ontology/obi/issues/1117 An insecticide resistance assay that is based on monitoring known mutations by PCR amplification of specific alleles to detect polymorphic or mutant alleles. VEuPathDB PMID: 1734929 metadata complete