has part RNA sequencing assay single-cell transcription profiling by RNA sequencing assay detection technique ultra high throughput single-cell combinatorial indexing RNA sequencing 3 assay sciRNAseq3 A single-cell transcription profiling by RNA sequencing assay that involves single cell combinatorial indexing ('sci-') to profile the transcriptomes of single cells or nuclei in an ultra high-throughput manor via changes to the method, as compared to sci-RNA-seq, that include extraction of nuclei directly from fresh tissue, use of hairpin ligation for the third level of indexing, optimization of enxymatic reactions, and use of dilution instead of FACS sorting. Sebastian Duesing sci-RNA-seq3 ultra high throughput single-cell combinatorial indexing RNA sequencing 3 assay https://pmc.ncbi.nlm.nih.gov/articles/PMC6434952/ Michelle Giglio metadata complete