has role has_role has role gene as virulence factor in pathogen gene mutant attenuated in host has role has_role has role Mus musculus Mus musculus 小家鼠 Ectromelia virus protein-coding gene of Ectromelia virus C13R - 12643 20130525 951519 Bin Zhao, Oliver He ECTVgp168 EVM167 NCBI-supplied PMID: 7747448; 9495531; 10725549; 18221766 WEB: http://www.ncbi.nlm.nih.gov/gene hypothetical protein protein-coding MUTATION: deletions of one to four genes of kelch family both individually (single mutants) and in different combinations (double, triple and quadruple mutants). It was shown that deletion of any of the three genes EVMO18, EVM027 or EVM167 resulted in reduction of 50% lethal dose (LD50) by five and more orders in outbred white mice infected intraperitoneally. Deletion of mousepox kelch-gene EVM150 did not influence the virus virulence. Two or more kelch-genes deletion also resulted in high level of attenuation, which could evidently be due to the lack of three genes EVM167, EVM018 and/or EVM027 identified as virulence factors. [PMID:19807023] 5100 virulence factor gene role