has role has_role has role gene as virulence factor in pathogen gene mutant attenuated in host cell line cell gene mutant attenuated in host cell gene mutant attenuated in host has role has_role has role HeLa cell HeLa cell HeLa 细胞 macrophage macrophage 巨噬细胞 Mus musculus Mus musculus 小家鼠 Brucella suis 1330 protein-coding gene of Brucella suis 1330 BRA0060 1164497 20140222 204722 BRA0060 Bin Zhao, Oliver He II NCBI-supplied PMID: 12271122; 16648257; 21763312 WEB: http://www.ncbi.nlm.nih.gov/gene protein-coding type IV secretion system protein VirB10 962 MUTATION: Mutants with polar and nonpolar mutations introduced in irB10 showed different behaviors in mice and in the HeLa cell infection assay, suggesting that virB10 per se is necessary for the correct function of this type IV secretion apparatus [PMID:11260139]. A B. abortus virB10 mutant showed a decrease of intracellular live bacteria comparable to that of the wild-type strain until 4 h after infection, indicating that a functional VirB system is not required for the short-term survival of Brucella inside macrophages. At later time points, the number of live virB10 mutants progressively decreased. Hence, the Brucella virB10 strain did not replicate, but rather was killed. Although the virB10 mutants are capable of short-term survival, they can not evade long-term degradation through fusion with lysosomes [PMID:11260139]. B abortus virB1 and virB10 mutants are unable to persist in mouse spleens after i.p. inoculation, suggest that attenuation in the animal model is due to an inability of these strains to grow intracellularly [PMID:11260139]. A B abortus virB10 mutant lost the ability to multiply in HeLa cells and was not recovered from the spleens of infected BALBc mice [PMID:11260139]. The non polar virB10 mutant was able to block the acquisition of cathepsin D, but was not able to translocate to the replication compartment [PMID:11260139]. The virB10 non-polar mutants were capable of avoiding interactions with the endocytic pathway but , diverging to wild-type Brucella, were unable to reach the endoplasmic reticulum to establish their intracellular replication niche and seemed to be recycled to the cell surface [PMID:11260139]. virulence factor gene role