has role has_role has role gene as virulence factor in pathogen has role has_role has role Brucella suis 1330 protein-coding gene of Brucella suis 1330 norD 1164688 20140217 204722 BRA0251 Bin Zhao, Oliver He II NCBI-supplied PMID: 12271122 WEB: http://www.ncbi.nlm.nih.gov/gene norD protein protein-coding 982 MUTATION: A mutant of Brucella suis bearing a Tn5 insertion in norD , the last gene of the operon norEFCBQD, encoding nitric oxide reductase, was unable to survive under anaerobic denitrifying conditions (more-than-5log reduction in viable counts). As a consequence of the norD mutation , NO might not be further reduced to N2O by the NO reductase and it could become toxic for the bacteria. The infection of resting macrophages showed that the norD mutant and the wild-type strain displayed similar rates of multiplication. On the contrary, activation of J774A.1 cells by LPS and IFN was accompanied by a more-thantenfold attenuation of the norD mutant at 48 h p. i. [PMID:16495577]. virulence factor gene role